Direct regeneration from leaf explants and genetic fidelity analysis of regenerates through ISSR markers in Kedrostis foetidissima: a medicinal climber

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Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00713-2
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Keywords: Genetic fidelity, ISSR, Leaf explants, n Kedrostis foetidissiman , Shoot regeneration


Abstract


Kedrostis foetidissima (Jacq.) Cogn. is a medicinal plant in Cucurbitaceae family. This plant has been in use since ancient days in folklore and Siddha systems of medicine. It is used to treat measles, diarrhea, eczema, urinary tract infections, asthma, and the common cold in children. It has many pharmacological activities such as anti-microbial, anti-diarrhoeal, anti-cancer, and antioxidant. Several phytochemical and pharmacological studies are reported on this plant, but scanty studies are reported for its in vitro propagation. So, the present study was envisaged to develop a protocol for leaf explants based direct regeneration. The MS medium supplemented with BAP, TDZ alone, and with IAA was used to induce the shoots from leaf explants. The MS medium amended with BAP (2.0 mg/L) combined with IAA (2.0 mg/L) induced the highest mean number of shoots after four weeks of first subculture. After three weeks of culture, the regenerated shoots have induced the highest rooting on half-strength MS medium augmented with IBA (1.0 mg/L). The regenerated plantlets were hardened in polycups containing sand, soil, and compost in a ratio of 1:1:1, then hardened (85%) in the greenhouse and shifted to the field with a good survival rate. The genetic homogeneity of in vitro regenerated plantlets was assessed by using ISSR markers, and no genetic variation was recorded. This is the first report on the direct regeneration of K. foetidissima from leaf explants and genetic fidelity analysis of regenerants with ISSR markers.


Genetic fidelity, ISSR, Leaf explants, n              Kedrostis foetidissiman            , Shoot regeneration


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References


Amghar I, Ibriz M, Ibrahimi M, Boudra A, Gaboun F, Meziani R, Abdelwahd R (2021) In vitro root induction from argan (Argania spinosa (L.) Skeels) adventitious shoots: influence of ammonium nitrate, auxins, silver nitrate and putrescine, and evaluation of plantlet acclimatization. Plants 10(6):1062


Amutha M (2017) Phytochemical Biological pharmacological evaluation of Kedrostis foetidissima Jacq Cogn and Plant mediated synthesis of metal nanoparticles. http://hdl.handle.net/10603/184784


Anywar G, Kakudidi E, Byamukama R, Mukonzo J, Schubert A, Oryem-Origa H (2020) Indigenous traditional knowledge of medicinal plants used by herbalists in treating opportunistic infections among people living with HIV/AIDS in Uganda. J Ethnopharmacol 246:112205


Aswathi NV, Thomas TD (2023) Direct and indirect shoot regeneration from leaf explants of Centratherum punctatum Cass., a wild ornamental plant. Sci Hortic 320:112201


Baghel S, Bansal YK (2017) In vitro regeneration of Guizotia abyssinica Cass. and evaluation of genetic Fidelity through R.A.P.D. markers. South Afr J Bot 109:294–307


Bulle M, Kota S, Rathakatla D, Aileni M, Kokkirala VR, Gadidasu KK, Abbagani S (2012) An efficient in vitro leaf-based regeneration and evaluation of genetic fidelity using ISSR markers in Woodfordia fruticosa (L.) Kurz. J Herbs Spices Med Plants 18(2):178–190


Chabukswar MM, Deodhar MA (2005) Rooting and hardening of in vitro plantlets of Garcinia indica Chois. Indian J Biotechnol 4:409–413


Chaitanya G, Suvarchala V, Ramakrishna D, Sunitha D, Shasthree T (2020) In vitro plant regeneration of Momordica cymbalaria Fenzl. and assessment of genetic Fidelity using ISSR primers. Research. J Biotechnol 15:2


Chakrvarty HL (1982) Fascicles of flora of India. Botanical Survey of India, Kolkata


Chandana BC, Nagaveni HC, Lakshmana D, Kolakar SS, Heena MS (2018) Role of plant tissue culture in micropropagation, secondary metabolites production and conservation of some endangered medicinal crops. J Pharmacogn Phytochem 7(3S):246–251


Chen X, Bao J, Guo J, Ding Q, Lu J, Huang M, Wang Y (2012) Biological activities and potential molecular targets of cucurbitacins: a focus on cancer. Anticancer Drugs 23(8):777–787





Choene M, Motadi LR (2012) Anti-proliferative effects of the methanolic extract of Kedrostis foetidissima in breast cancer cell lines. Mol Biol 1(2):1–5. https://doi.org/10.4172/mbl.1000107


Daud N, Faizal A, Geelen D (2013) Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red L.E.D. light. In Vitro Cell Dev Biol Plant 49(2):183–190


Deepa AV, Thomas TD (2022) High-frequency direct shoot induction from leaf explants of Pogostemon quadrifolius (Benth.) F. Muell.: an ethnomedicinal herb. In Vitro Cell Dev Biol Plant 58:321–329. https://doi.org/10.1007/s11627-022-10265-w


Devendra NK, Rajanna L, Sheetal C, Seetharam YN (2008) In vitro clonal propagtion of Trichosanthes cucumerina L. var. cucumerina. Plant Tissue Cult Biotechnol 18(2):103–111


Doyle JJ, Doyle JL (1990) Isolation of plant D.N.A. from fresh tissue. Focus 12(13):39–40


Elavazhagan T (2016) Studies on phytochemical antimicrobial and anticancer activity against Kedrostis Foetidissima. Jacq Cogn. http://hdl.handle.net/10603/230788


Giday M, Asfaw Z, Elmqvist T, Woldu Z (2003) An ethnobotanical study of medicinal plants used by the Zay people in Ethiopia. J Ethnopharmacol 85(1):43–52. https://doi.org/10.1016/S0378-8741(02)00359-8


Gullón B, Lú-Chau TA, Moreira MT, Lema JM, Eibes G (2017) Rutin: a review on extraction, identification and purification methods, biological activities and approaches to enhance its bioavailability. Trends Food Sci Technol 67:220–235


Jogam P, Sandhya D, Shekhawat MS, Alok A, Manokari M, Abbagani S, Allini VR (2020) Genetic stability analysis using DNA barcoding and molecular markers and foliar micro-morphological analysis of in vitro regenerated and in vivo grown plants of Artemisia vulgaris L. Ind Crops Prod 151:112476. https://doi.org/10.1016/j.indcrop.2020.112476


John Britto S (2019) The Flora of Central and North Tamil Nadu. Part-2. The Rapinat Herbarium. St. Joseph’s College (Autonomous),Tiruchirappalli (India)


Kabir AH, Sarker KK, Sharmin SA, Islam MS, Alam MF (2008) Callus induction and plantlet regeneration in Abelmoschus esculentus (L.) Moench. J Agric Technol 4:193–204


Kaur K, Kaur K, Bhandawat A, Pati PK (2021) In vitro shoot multiplication using meta-topolin and leaf-based regeneration of a withaferin a rich accession of Withania somnifera (L.) Dunal. Ind Crops Prod 171:113872








Krishna H, Alizadeh M, Singh D, Singh U, Chauhan N, Eftekhari M, Sadh RK (2016) Somaclonal variations and their applications in horticultural crops improvement. 3 Biotech 6(1):1–18


Kunthavi B, Malarvizhi M, Poongodi Kandhimathi AS (2018) Retrospective study of leaves of Kedrostis foetidissima in siddha medicine. Int J Curr Res Biol Med 3(5):18–21. https://doi.org/10.22192/ijcrbm.2018.03.05.004


Mali AM, Chavan NS (2016) In vitro rapid regeneration through direct organogenesis and ex-vitro establishment of Cucumis trigonus Roxb.—Anunderutilized pharmaceutically important cucurbit. Ind Crops Prod 83:48–54





Mood K, Jogam P, Sirikonda A, Shekhawat MS, Rohela GK, Manokari M, Allini VR (2022) Micropropagation, morpho-anatomical characterization, and genetic stability studies in Lippia javanica (Burm. f.) Spreng: a multipurpose medicinal plant. Plant Cell, Tissue and Organ Culture 150:427-437


Mugomeri E, Chatanga P, Raditladi T, Makara M, Tarirai C (2016) Ethnobotanical study and conservation status of local medicinal plants: towards a repository and monograph of herbal medicines in Lesotho. Afr J Tradit Complement Altern Med 13(1):143–156


Nalumansi P, Godwin A, Kamatenesi-Mugisha M (2014) Medicinal plants used in paediatric health care in namungalwe sub county. iganga district, Uganda


Nirmala J, Pandian R (2013) Morphological and systematic studies on Kedrostis foetidissima (Jacq.) Cogn. Int J Curr Microbiol Appl Sci 2(10):560–571


Njoroge GN, Newton LE (1994) Edible and poisonous species of Cucurbitaceae in the Central Highlands of Kenya. J East Afr Nat Hist 83(2):101–115


Pandey S, Patel P, Prasad A et al (2020) Assessment of direct shoot organogenesis and genetic Fidelity in Solanum viarum Dunal—a commercially important medicinal plant. In Vitro Cell Dev Biol Plant 56:538–547. https://doi.org/10.1007/s11627-020-10073-0


Petrova M, Zayova E, Yankova E, L. I. N A, Baldzhiev G (2011) Plant regeneration from callus culture of Arnica montana. Roman Biotechnol Lett 16(1):92–97


Pillai GS, Martin G, Raghu AV, Lyric PS, Balachandran I, Ravindran PN (2008) Optimizing culture conditions for in vitro propagation of Trichosanthes cucumerina L.: an important medicinal plant. J Herbs Spices Med Plants 14(1–2):13–28





Priyavardhini S, Gowri SS, Vasantha K, Umadevi M (2008) Antibacterial activity of stem and leaf extract of Kedrostis foetidissima (Jacq.) Cogn. Anc Sci Life 28(2):10


Priyavardhini S, Vasantha K, Soris T, P., Mohan VR (2012) Antifungal activity of Kedrostis foetidissima (Jacq.) Cogn. Int J Pharm Tech Res 4(1):44–48


Raji MR, Farajpour M (2021) Genetic fidelity of regenerated plants via shoot regeneration of muskmelon by inter simple sequence repeat and flow cytometry. J Saudi Soc Agric Sci 20(2):88–93


Renner SS, Pandey AK (2013) The Cucurbitaceae of India: accepted names, synonyms, geographic distribution, and information on images and D.N.A. sequences. PhytoKeys. https://doi.org/10.3897/phytokeys.20.3948


Rios JL, Escandell JM, Recio M (2005) New insights into the bioactivity of cucurbitacins. Stud Nat Prod Chem 32:429–469. https://doi.org/10.1016/S1572-5995(05)80062-6


Rohela GK, Jogam P, Mir MY, Shabnam AA, Shukla P, Abbagani S, Kamili AN (2020) Indirect regeneration and genetic fidelity analysis of acclimated plantlets through SCoT and ISSR markers in Morus alba L. cv. Chinese white. Biotechnol Rep 25:e00417





Sandhya D, Jogam P, Manokari M, Shekhawat MS, Jadaun JS, Allini VR, Abbagani S (2021) High-frequency in vitro propagation and assessment of genetic uniformity and micro-morphological characterization of Origanum majorana L.–a highly traded aromatic herb. Biocatal Agric Biotechnol 34:102024


Savitikadi P, Jogam P, Rohela GK, Ellendula R, Sandhya D, Allini VR, Abbagani S (2020) Direct regeneration and genetic fidelity analysis of regenerated plants of Andrographis echioides (L.)-an important medicinal plant. Ind Crops Prod 155:112766. https://doi.org/10.1016/j.indcrop.2020.112766


Sen MK, Nasrin S, Rahman S, Jamal AHM (2014) In vitro callus induction and plantlet regeneration of Achyranthes aspera L., a high value medicinal plant. Asian Pac J Trop Biomed 4(1):40–46


Shiji R, George J, Sunitha S, Vandhana A, Muthuraj R (2014) Effect of N.A.A. and I.B.A. on in vitro regeneration and hardening in cassava (Manihot esculenta Crantz). J Root Crops 40(2):12–20


Sivaprakash V (2016) Antidiarrhoeal activity of Kedrostis Foetidissima leaf extract on experimentally induced diarrhoea in mice, Doctoral dissertation. Nandha College of Pharmacy, Erode








Tilkat E, Onay A, Yıldırım H, Ayaz E (2009) Direct plant regeneration from mature leaf explants of pistachio, Pistacia vera L. Sci Hortic 121(3):361–365. https://doi.org/10.1016/j.scienta.2009.02.007


Tripathy SK, Ithape DM (2020) High-throughput in vitro culture system targeting genetic transformation in sugarcane. J Crop Sci Biotechnol 23(4):325–335

 


Acknowledgements


The authors are thankful to Head, Department of Botany and Biotechnology, Kakatiya University, for providing facilities. K.S. is grateful to the principal, Pingle Govt. College for Women (A), Hanumakonda, for support and encouragement.


Author Information


Saritha Kommidi
Plant Tissue Culture and Molecular Taxonomy Laboratory, Department of Botany, Kakatiya University, Warangal, India